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TECHNICAL SERVICE GUIDE: Immunohistochemistry

Catalog #: Lot #:

PROBLEM AND PREVIOUS EXPERIENCE

1. What is the specific problem you are experiencing?

2. Did this same vial of product work in the past? What were the results?

3. Did other lots of this product work in the past? Which lots? What were the results?

STAINING PATTERN

1. What pattern of staining was expected and what was observed?

CONTROLS

1. Has the primary antibody （used in the immunohistochemistry） been used successfully in any other applications （Western Blot, Immunoprecipitation,Immunohistochemistry with different method of fixation） with the same sample?

2. What positive and/or negative controls were done and what were the results?

SAMPLES AND FIXATION

1. From which species （animal） was the sample?

2. What type of sample was stained （frozen tissue, paraffin-embedded tissue, tissue culture cells）?

3. What method of fixation was used （acetone, formalin, methanol）?

4. Was Antigen-Retrieval performed? If so, by what method （heat treatment, enzyme digestion）?

BLOCKING

1. What were the blocking conditions （blocking solution, species of serum, length of incubation）?

PRIMARY ANTIBODY

1. At what dilution（s） was the primary antibody used and what was the diluent?

2. What were the incubation conditions （time, temperature） for the primary antibody?

3. At what temperature has the primary antibody been stored? Has it ever been frozen?

SECONDARY ANTIBODY

1. From which company was the secondary antibody obtained and what type of secondary was being used （goat anti-rabbit, rabbit anti-mouse, etc.） and what

secondary antibody conjugate was used （biotin, FITC, Rhodamine, Texas Red）?

2. At what dilution（s） was the secondary antibody used and what was the diluent?

上海起福生物科技有限公司
：楊建輝
：
辦公：
*8006

:
:http://www.shluck.com.cn/
地址：上海市浦東新區(qū)繡川路561號1102室